Immunocytochemistry/Immunofluorescence analysis of PrP in U251 cells using PrP Recombinant Monoclonal Antibody (Product # MA5-47985). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with PrP Recombinant Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom PrP Recombinant Monoclonal Antibody, DAPI, merged channels and an isotype control. The isotype control was stained with Mouse IgG1 Isotype Control Antibody (Product # MA5-47823) followed by Alexa Fluor488 secondary antibody.